| 质粒名称: | pPlin5-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000756 |
| 产品用途: | Expresses Plin5-mScarlet3-H in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Plin5, mScarlet3-H |
| 物种来源: |
H.sapiens, Synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pPlin5-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pPlin5-mScarlet3-H (aka mYongHong)载体质粒基本信息
pPlin5-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pPlin5-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
载体图谱.png)
pPlin5-mScarlet3-H (aka mYongHong)质粒载体简介
pPlin5-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pPlin5-mScarlet3-H (aka mYongHong) 6024 bp DNA circular SYN 30-DEC-2024
DEFINITION TSN20241013-0571-00010_756_20241014-BAN4_G03_G03_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6024)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6024
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 124..327
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 362..1744
/codon_start=1
/label=Perilipin5
/translation="EEEAAQIPRSSVWEQDQQNVVQRVVALPLVRATCTAVCDVYSAAK
DRHPLLGSACRLAENCVCGLTTRALDHAQPLLEHLQPQLATMNSLACRGLDKLEEKLPF
LQQPSETVVTSAKDVVASSVTGVVDLARRGRRWSVELKRSVSHAVDVVLEKSEELVDHF
LPMTEEELAALAAEAEGPEVGSVEDQRRQQGYFVRLGSLSARIRHLAYEHSVGKLRQSK
HRAQDTLAQLQETLELIDHMQCGVTPTAPACPGKVHELWGEWGQRPPESRRRSQAELET
LVLSRSLTQELQGTVEALESSVRGLPAGAQEKVAEVRRSVDALQTAFADARCFRDVPAA
ALAEGRGRVAHAHACVDELLELVVQAVPLPWLVGPFAPILVERPEPLPDLADLVDEVIG
GPDPRWAHLDWPAQQRAWEAEHRDGSGNGDGDRMGVAGDICEQEPETPSCPVKHTLMPE
LDF"
regulatory 1754..1763
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1760..2449
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
polyA_signal 2571..2692
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2699..3154)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3181..3285
/gene="bla"
/label=AmpR promoter
promoter 3287..3644
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3495..3630
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3679..4473
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4705..4752
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5081..5669
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer join(5844..6024,1..123)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
61 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
121 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
181 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
241 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
301 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcatgag
361 cgaggaggag gctgctcaga tccccagaag cagcgtgtgg gagcaggacc agcagaacgt
421 ggtgcagaga gtggtggccc tgcctctggt gagagctaca tgcaccgccg tgtgcgacgt
481 gtacagcgct gctaaagaca gacaccccct gctgggcagc gcttgtagac tggctgagaa
541 ttgcgtgtgc ggcctgacca ccagagctct ggatcacgct cagcccctgc tggaacacct
601 gcagcctcag ctggctacca tgaattccct ggcttgcaga ggcctggaca agctggaaga
661 gaaactgccc ttcctgcagc agccctccga gacagtggtg acaagcgcta aagacgtggt
721 ggcctcctcc gtgaccggag tggtggatct ggctagaaga ggcagaagat ggagcgtgga
781 actgaagagg agcgtgagcc acgccgtgga tgtggtgctg gaaaagagcg aggagctggt
841 ggaccatttt ctgcccatga ctgaggaaga gctggccgct ctggccgctg aagctgaagg
901 acctgaggtg ggaagcgtgg aagaccagag aagacagcag ggatacttcg tgagactggg
961 atccctgagc gccagaatca gacacctggc ctacgagcac tccgtgggaa aactgagaca
1021 gagcaagcac agagcccagg acaccctggc tcagctgcag gaaaccctgg agctgattga
1081 ccacatgcag tgcggcgtga cccctacagc tcctgcttgt cctggcaaag tgcacgagct
1141 gtggggcgaa tggggccaga gacctcctga aagcagaaga agaagccagg ccgagctgga
1201 gaccctggtg ctgtctagat ccctgactca ggagctgcag ggaacagtgg aggccctgga
1261 aagcagcgtg agaggactgc cagccggagc tcaggaaaag gtggctgagg tgagaagaag
1321 cgtggatgcc ctgcagaccg ccttcgctga tgctagatgc ttcagagacg tgcccgccgc
1381 tgctctggct gaaggaagag gaagagtggc ccacgcccat gcttgtgtgg acgaactgct
1441 ggagctggtg gtgcaggctg tgcctctgcc ttggctggtg ggacctttcg ctcctattct
1501 ggtggagagg cccgagcctc tgcctgatct ggctgatctg gtggatgaag tgatcggcgg
1561 ccctgatccc agatgggctc atctggactg gcctgctcag cagagggctt gggaagctga
1621 gcacagagac ggatccggca acggagacgg agacagaatg ggagtggccg gagatatttg
1681 cgagcaggag cctgaaacac ccagctgccc agtgaagcac accctgatgc ccgaactgga
1741 cttcccaccg gtcgccacca tggatagcac cgaggcagtg atcaaggagt tcatgcggtt
1801 caaggtgcac atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
1861 gggccgcccc tacgagggca cccagaccgc caagctgagg gtgaccaagg gtggccccct
1921 gcccttctcc tgggacatcc tgtcccctca gttcatgtac ggctccaggg ccttcacgaa
1981 gcaccccgcc gacatccccg actactggaa gcagtccttc cccgagggct tcaagtggga
2041 gcgcgtgatg aacttcgagg acggcggcgc cgtgtccgtg gcccaggaca cctccctgga
2101 ggacggcacc ctgatctaca aggtgaagct ccgcggcacc aacttccctc ctgacggccc
2161 cgtaatgcag aagaagacaa tgggctggga agcatccacc gagcggttgt accccgagga
2221 cgtcgtgctg aagggcgaca ttaagcatgc cctgcgcctg aaggacggcg gccggtacct
2281 ggcggacttc aagaccacct acagggccaa gaagcccgtg cagatgcccg gcgccttcaa
2341 catcgaccgc aagttggaca tcacatccca caacgaggac tacaccgtgg tggaacagta
2401 cgaacgctcc gtggcccgcc actccaccgg cggctccggt ggctcctaaa gcggcccgac
2461 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
2521 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
2581 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
2641 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa
2701 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
2761 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
2821 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
2881 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
2941 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
3001 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
3061 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
3121 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
3181 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
3241 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
3301 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
3361 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
3421 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
3481 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
3541 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
3601 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
3661 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
3721 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
3781 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
3841 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
3901 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
3961 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
4021 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
4081 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
4141 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
4201 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
4261 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
4321 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
4381 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
4441 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
4501 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
4561 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
4621 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
4681 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
4741 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
4801 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
4861 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
4921 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
4981 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
5041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
5101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
5161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
5221 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
5281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
5341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
5401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
5461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
5521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
5581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
5641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
5701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
5761 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
5821 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
5881 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
5941 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
6001 gtacatcaag tgtatcatat gcca
//
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